Evaluation of cardiac differentiation potential of menstrual blood- derived stem cells seeded in biocompatible bombyx mori silk fibroin scaffold in co-culturing with neonatal rat cardiomyocytes

Maryam Rahimi,1,* Arash babaei,2 Marzieh atshan ,3 Mitra mohamadi ,4

Abstract

Introduction

Cardiovascular disorders are leading cause of million deaths each year which reveals the significance of current limitations in common therapies. recently , much attention has been given to the artificial cardiac microenvironment and three-dimensional (3d) scaffolds have been utilized as potential therapies for treating heart failure following myocardial infarction (mi).

Methods

In this study, we investigate cardiac differentiation capacity of menscs seeded bombyx mori silk fibroin scaffold in co-culture with neonatal rat cardiomyocytes . the expression of the putative myogenic cells at mrna and protein levels was determined by immunofluorescent staining and real-time quantitative pcr.

Results

Our data revealed that differentiated menscs seeded silk scaffold in co-culture with neonatal rat cardiomyocytes acquired some features of cardiomyocytes and showed upper levels of mrna/protein of late stage cardiac markers under co-culture with neonatal rat cardiomyocytes evidencing the key role of chemical factors and stimulation signal of extracellular matrix in cardiac development of stem cells. differentiated menscs seeded silk scaffold under co-culture with neonatal rat cardiomyocytes showed remarkable expression of connexin-43,tnnt2 and actn-2 at three gene and protein levels,

Conclusion

This research demonstrates that menscs have higher capability to be differentiated toward cardiomyocyte in silk scaffold under co-culture with neonatal rat cardiomyocytes . furthermore, suggests the therapeutic potential of silk scaffold-seeded menscs in the treatment of ischemic heart diseases.

Keywords

Menstrual blood, silk scaffold, cardiac tissue engineering, co-culture