Evaluation of immunomodulatory effect of oral administration of aloe vera extracted polysaccharides on the immunization profile of vaccinated mice with e7d vaccine

Mahboubeh Nikookalam mirzaee,1,* Zeinab salehian,2 sahar mirzaee,3 Mehdi mahdavi,4 Ramak ajideh,5 Mohammadhossein yazdi,6

1. Department of Biotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad Uni
2. Department of Biotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad Uni
3. Department of Biotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad Uni
4. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
5. Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of
6. Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of

Abstract

Introduction

In this work, the effect of oral administration of aloe vera extracted polysaccharides was investigated on the immunization profile of e7d vaccine. mannose containing polysaccharide in aloe vera mucilage absorbed after oral feeding of mice and remained unchanged in the blood stream. subsequently, it can act as an immunomodulator which is induced both th1 and th2 immune response.

Methods

60c57bl/6 mice were divided into six groups as follow: group 1, e7d-alum-gel; group 2, e7d-alum; group 3, e7d-montanid -gel; group 4, e7d-montanid; group 5 and group 6,control-gel and pbs respectively. the oral feeding of aloe vera gel with 0.05% polysaccharide was done for a period of 30 days. the mice were immunized with e7d vaccine three times on day 0, 14 and 28th. then tumor was implanted subcutaneously and 2 weeks later, the serum samples of the mice were collected to evaluate the cytokines and antibodies levels by elisa assay

Results

Immunization of mice with e7d-alum-gel significantly increased the ifn-γ and il-4 immune response levels in comparison to other vaccinated groups. the group which was received just aloe vera gel demonstrated the greatest reduction in proportion of tumor growth.

Conclusion

Whereas mannose containing plant compounds like acemannan and glucomannan induces both th1 and th2 immune responses, perhaps it can be used as an adjuvant element without serious concern about the toxicity

Keywords

E7d vaccine, adjuvant, aloe vera polysaccharide