1. Tabriz university of medical science 2. Tabriz university of medical science 3. Tabriz university of medical science
Abstract
Introduction
Chrysin is a natural flavonoid found abundantly in honey, gum, and many plant extracts. it rep¬resents anti-cancer features. being loaded with plga-peg increases chrysin’s solubility and drug tolerance. cyclin d1, a regulatory protein in cell proliferation cycle, has over-ex¬pression in breast cancer tumors. this study aimed to evalu¬ate the effect of chrysin-loaded plga-peg on cyclin d1 gene expression as a marker of tumor progression.
Methods
T47d cell line was cultured in rpmi1640 with 10% fbs and was exposed to various concentrations of pure chrysin and chrysin-loaded plga-peg (36, 56 and 76 μ m) for 24, 48, and 72 hours. proton nuclear magnetic resonance (hnmr), fourier-transform infrared spectroscopy (ft-ir), and scanning electron microscopy (sem) were used to delineate the chrysin loading. in vitro cytotoxicity and ic50 of pure and nano-chrysin were studied by the mtt assay. the rna was exploited and cytotoxic effects of chrysin on cyclin d1 gene expression were studied by real-time pcr.
Results
Cytotoxic effect of nano-chrysin was higher than pure chrysin. the toxic effect of chrysin was increased with drug dose elevation, demonstrating the dose-dependent association. both pure chrysin and nano-chrysin exposure decreased cyclin d1 gene expression.
Conclusion
The nano-chrysin therapy is developed a novel method that could increase cytotoxicity to cancer cells with¬out damaging the normal cells and would be promising in breast cancer therapy.
