Investigation the effect of low intensity pulsed ultrasound (lipus) on proliferation of g 292 osteosarcoma cells on 3d porous plga/β-tcp scaffold within perfusion bioreactor

Mohammadreza Safari hajat aghaei,1 Ghassem amoabediny,2,* Hadi tabesh,3 Behrouz zandieh doulaby,4

1. Faculty of New Sciences and Technologies (FNST), University of Tehran,Tehran, Iran
2. Faculty of Chemical Engineering, college of engineering, University of Tehran, Tehran, Iran
3. Assistant professor, faculty of New Sciences and Technologies (FNST), University of Tehran, Tehran, Iran
4. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam,Vrije University of Amsterdam

Abstract

Introduction

Investigation the effect of low intensity pulsed ultrasound (lipus) on proliferation of g 292 osteosarcoma cells on 3d porous plga/β-tcp scaffold within perfusion bioreactor

Methods

The cells were seeded on plga/β-tcp porous scaffold (20mm*7.5mm) and cultured for 3 and 7 days in static and dynamic bioreactor (37 ºc, 5% co2, 95% humidity) with 20 min/day lipus treatment (frequency 1.5 mhz and intensity 30 mw/cm3). the cells were evaluate for attachment (sem) and viability (mtt assay) after 3 and 7 days of culture with and without lipus treatment.

Results

The mtt assay analysis results shown 1.44 and 0.7 fold increase in cell proliferation in 3 and 7 days of static culture and also 1.16 and 0.38 fold increase in cell proliferation in 3 and 7 days of dynamic culture by ultrasound treatment respectively. homogeneity of cells distribution on the surface of scaffold in dynamic culture by using lipus were seen.

Conclusion

Our data shows enhancement on proliferation of g 292 osteosarcoma cells and increasing the cell number in static and dynamic culture. stimulation by lipus offer a tool for applying controlled mechanical loading on cell ingrowth and has potential to use in 3d bone tissue engineering and it can be used when the use of grows factors are limited. it can be used for targeted differentiation of stem cells

Keywords

Ultrasound perfusion bioreactor shear stress bone cells