Hepatoprotective effects of magnesium sulfate on pentylenetetrazol-induced seizures in male wistar rats

Naghmeh Moghimi,1,* Akram eidi,2 pejman mortazavi,3 ali haeri rohani,4

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Department of Pathology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Magnesium is the second most abundant cation (after potassium) in the cell and plays an important role in various biological functions, including cell cycle, channel regulation, atpase activity, metabolic regulation, etc. magnesium deficiency causes an increased incidence of cardiovascular diseases, including hypertension, stroke and atherosclerosis, and gastrointestinal disorders, such as loss of appetite, nausea, and vomiting. in the present study, the effect of magnesium sulfate on pentylenetetrazole (ptz)-induced seizures was investigated in adult male wistar rats.

Methods

The rats were randomly divided into 8 groups: normal control, magnesium sulfate (0.05, 0.1, and 0.2 g/kg intragastrically, daily) alone, epileptic control rats (ptz, 35 mg/kg, i.p.), magnesium sulfate (three doses as above) together with ptz, and treatment was performed accordingly. administration of magnesium sulfate was started 1.5 h before the first dose of ptz. the rats were sacrificed on day 29 and parameters of oxidative stress, superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx) and malondialdehyde (mda) levels, were measured in liver homogenate.

Results

Our data showed that the activity of antioxidant enzymes (cat, sod, and gpx) decreased significantly in the epileptic control group compared to the normal control group. on the other hand, the level of mda was increased significantly in the epileptic control group as compared to the normal control group. our results showed that administration of magnesium sulfate increased the levels of antioxidant enzymes, including sod, cat, gpx, while decreased mda levels.

Conclusion

These results indicate that magnesium sulfate is effective in providing protection against oxidative stress induced by ptz.

Keywords

Magnesium sulfate; pentylenetetrazole; seizure; rat