Breast cancer (bc) is increasing in the developing countries. estrogens are supposed to play roles in bc initiation and progression/promotion. most of bc cells express estrogen receptors α (er α) and β (er β).
chrysin, a natural flavonoid found in honey, stimulates apoptosis and presents anti-cancer features in human cells. the current study attempts to distinct whether chrysin nanoparticles alter estrogen receptors’ expression.
Methods
T47d cell line was cultured in rpmi1640 with 10% fbs. after synthesis of plga-peg and drug loading, the structure and chrysin loading percentage were delineated using proton nuclear magnetic resonance (hnmr), fourier-transform infrared spectroscopy
(ft-ir), and scanning electron microscopy (sem) and t47d cells were exposed to three doses of free chrysin and chrysin-loaded plga-peg (36, 56 and 76 μm) in the exponential phase of growth. mtt assay at 24, 48 and 72h after treatment was used to evaluate the cytotoxic effect of free chrysin and chrysin-loaded plga-peg.
finally, the cells rna was exploited by rnx-plus kit and the levels of er α and er β gene expression were measured by real-time pcr.
Results
Both pure and nano-capsulated chrysin diminished the tumor proliferation. erα gene expression was decreased, and erβ gene expression was increased significantly in a dose-dependent manner.
Conclusion
Chrysin nanoparticles present a safe method to control estrogen receptor gene expression in human breast cancer cells. more investigations are warranted before clinical implementations.
Keywords
Er α, er β, gene expression, chrysin nanoparticles, breast cancer
