It is known that application of mesenchymal stem cells is an effective treatment in many diseases. despite of cell therapy benefits, it faces to several problems. the cells viability decreased after injection into the body because of factors such as low serum, hypoxia, heat shock and oxygen free radicals. to increase the resistance of cells against toxic stresses, the present study investigated the effects of preconditioning with hydrogen peroxide (h2o2) and serum deprivation on cell viability and apoptosis.
Methods
Mesenchymal stem cells from bone marrow were cultured and preconditioned with serum deprivation and h2o2 for 6, 12, 24 and 48 hours in 6 groups (i: control, ii: 5μm h2o2, iii: 10μm h2o2, iv: 5%fbs, v: 5μm h2o2+5%fbs and vi: 10μm h2o2+5%fbs). after these time periods of treatment, the mscs were exposed to lethal dose of h2o2 (300 μm) for 24 hours. then, tryptan blue staining was conducted to evaluate the cell viability. also, tunel assay was done to study the cell apoptosis.
Results
according to our data, the cell viabilityingroupsv and vi, which were both preconditions at all times were significantly increased compared to control group. moreover, cell apoptosis of v and vi groups was significantly lower than control group after 12, 24 and 48 hours (p < 0.01).
Conclusion
Our findings suggest that preconditioning with 5μm h2o2+5%fbs and 10μm h2o2+5%fbs can improve the cell viability as well as lead to decline in cell apoptosis.
our findings suggest that preconditioning with 5μm h2o2+5%fbs and 10μm h2o2+5%fbs can improve the cell viability as well as lead to decline in cell apoptosis.
our findings suggest that preconditioning with 5μm h2o2+5%fbs and 10μm h2o2+5%fbs can improve the cell viability as well as lead to decline in cell apoptosis.
our findings suggest that preconditioning with 5μm h2o2+5%fbs and 10μm h2o2+5%fbs can improve the cell viability as well as lead to decline in cell apoptosis.
