Isolation of dehalogenase producing bacteria from a marine environment that can degrade 2,2-dichloropropionate (2,2-dcp)

Mona Kiminezhad,1,* Parisa kiminezhad,2

Abstract


Introduction

Most halogenated compounds are major environmental pollutants.2,2-dichloropropionic acid is an organochloride herbicide and plant growth regulator which used as herbicide. a group of microorganisms are involved in the biodegradation of many important halogenated pollutants.

Methods

In current experiment successfully isolated bacterium mk1 by partial biochemical and 16s rrna sequencing method was isolated with 2,2dcp (2, 2-dichloropropionic acid) as the sole carbon and energy source from marine.

Results

This bacterium has the ability to degrade 2,2-dcp (2,2-dichloropropionic acid) in the presence of 8% and 16% of nacl as elevated levels of chloride ion release were detected using halide ion assay with maximum chloride ion liberation recorded at 0.898 mm after 40hours of exponential growth in 10mm 2,2-dcp

Conclusion

Bacterium strain mk1 was able to attain optimal growth in this minimal medium being metabolized as the primary carbon and energy source with a doubling time of 2.55hours

Keywords

2,2-dichloropropionic acid,16srrna, pcr amplification