1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran Universi 2. Department of Hematology, Tarbiat Modares University, Tehran, Iran 3. Department of Hematology, Tarbiat Modares University, Tehran, Iran
Abstract
Introduction
Colorectal cancer is one of the most common epithelial cancers in the world. many studies have been conducted on the inhibitory effects of probiotics on cancer and other diseases.
Methods
Caco2 cells were cultured in dmem containing 25% supernatant of la or ra .then proliferation and survival of cells were studied by direct counting, mtt assay and invasion potential were assessed by chamber assay. the changes in expression of mmp9 and mmp12 genes in the presence of bacterial supernatants was investigated by qrt- pcr .
Results
Report showed significant difference in proliferation of caco2 cells after exposure to the supernatants. in the la group was a reduction in cellular invasion. (** = 0.0074)
analysis of the expression levels of mmp9 showed a sign decrease in the transcript level of mmp9 in both supernatants and increase in mmp12 in la .
Conclusion
Matrix metalloproteinase (mmps) play a prominent role in cancer cell invasion and dissemination. expression levels of mmp--9 correlate with worse outcomes; mmp-12 expression appears to be protective.
la supernatant had a greater effect on reducing cell invasion or mmp9 gene expression, and increasing the expression of mmp12 gene or cancer recovery. the acidity of the outside of the cancer cells due to aerial glycolysis, it seems that la, which tolerates acidic conditions, is a more appropriate option for subsequent experiments.
Keywords
Lactobacillus acidophilus or rhamnosus,caco2 cell line, invasion ,proliferation,colorectal cancer
