Benzoquinone(bq) is one of benzene metabolite after entering body, its prolonged presence in body leads to blood disorders such as leukemia. among cells in bm niches are mesenchymal stem cells(mscs) and hematopoietic cells(hscs). mscs are regulators of hematopoiesis in bm that affecting self-proliferation, differentiation and self-renewanation of hscs. jag1 and wnt5a genes are involved in hematopoiesis pathway, which secretes from stromal cells to niche and regulate hscs. in this study, effect of 5μm benzoquinone (concentration of viability activity of mscs) on the expression of jag1 and wnt5a genes was investigated.
Methods
Mscs were isolated from bm aspiration and in the third passage were analyzed by flow-cytometry and bone differentiation. cells were then subjected to benzoquinone for 24 hours. expression of jag1 and wnt5a genes under benzoquinone treatment was performed by quantitative rt-pcr.
Results
Results indicate that 5μm benzoquinone leads to an increase in vital activity of mscs. also, treatment of mscs at 5μm concentration resulted in significant increase in expression of jag1 and wnt5a genes in these cells more than untreated controls.
Conclusion
Wnt5a gene is a non-canonical wnt pathway ligand. wnt messenger pathways play important role in regulating activity of hscs. jag1 gene is ligand of notch pathway, which ultimately causes differentiation and molding of hscs. thus, changes in the expression of these genes cause disbalancing of hematopoietic niche and cause bleeding disorder and eventually leukemia. therefore, bq is effective in hscs changes by altering secretion of mscs in the bm niche.
