This study investigated the in vitro release of vancomycin and amphotericin b fromnovel mocuadhesive chitosan nanofibers. for the electrospinning of biodegradable mocuadhesive membranes, the chitosan (cs) with low molecular weight, dd: 91.2 %, viscosity: 220mpa. sand poly ethylene oxide (peg) with a ratio of 80:20 was used. (cs/peg) were separately dissolved in acetic acid. chitosan and poly ethylene glycol as a main polymer and auxiliary polymer. they werethen electrospun into mocuadhesive structured membranes, with chitosan-4-thio-butyl-amidine as a mocuadhesive agent.
Methods
Chitosan (cs) (low molecular weight, dd: 91.2 %, viscosity: 220 mpa) was purchased from
hocheon co. (china). poly ethylene glycol (peg), glacial acetic acid, acetone, absolute
ethanol, naoh, kh2po4, vancomycin, amphotericin-b from sigma aldrich ( usa).
the electrospinning setup utilized in this study consisted of a syringe and needle (the internal diameter 1.2 mm), a ground electrode, an aluminum sheet, and a high voltage supply. electrospinning was done by a syringe pump with a volumetric flow rate of 1.2 ml/h.
Results
The experiment showed that biodegradable mocuadhesive nanofibrous membranes released high concentrations of vancomycin and amphotericin b for 2 day, respectively.
Conclusion
In the present study, a new chitosan-based mucoadhesive nanofiber mats have been
proposed for oral diseases treatment. the bioactivity of vancomycin ranged
from 90% to 95%. the antibacterial and anti-fungal experiments proved the efficiency of
nanofibers. in addition, results indicated that the nanofibers were functionally active in the
treatment of oral diseases.
Keywords
Chitosan, electrospinning, drug delivery, nanofibrous
