Preconditioning of bone marrow-derived mesenchymal stem cells by adiporon potentiates cell migration ability, in vitro.

Sara Malih,1,* Rezvan najafi,2 Massoud saidijam,3 Kamran mansouri,4

1. Research center for molecular medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2. Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3. Research center for molecular medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
4. Medical Biology Research Center, Kermanshah University of Medical, Sciences, Kermanshah, Iran.

Abstract

Introduction

Mesenchymal stem cells (mscs) have represented a great potential in recent stem cell-based therapies as well as regenerative medicine. due to low viability of transplanted cells novel strategies are needed to improve viability and robust migration of the cells. herein we studied the effect of adiporon on factors involved in migration and angiogenesis of rat bone marrow-derived mscs.

Methods

Mscs were treated with different concentrations of adiporon for 24 h. the expression level of hypoxia-inducible factor-1 (hif-1), vascular endothelial growth factor (vegf), matrix metalloproteinase-2 (mmp-2) and mmp-9, ang-2 (angiopoietin 2) and ang-4 were assessed by real-time pcr, compared to untreated mscs. gelatin zymography assay was applied to investigate the protease activity of mmp-2 and mmp-9. the tube formation potential of mscs were observed by three dimentional vessel formation assay.

Results

The real-time pcr results indicated that the expression of hif-1, vegf, mmp-2 and mmp-9 were higher in adiporon- treated mscs compared to control groups when ang-2/4 didn’t show any significant change. the activity of mmp2/9 enzymes were increased in treated samples with adiporon compared to control group. adiporon preconditioning didn’t show any significant change in huvecs tube formation and were similar to control group.

Conclusion

Preconditioning of mscs with adiporon prior to transplantation could enhance the migration via overexpression and activation of mmp-2/9.

Keywords

Adiporon, mesenchymal stem cell, matrix metalloproteinases, migration