1. Ferdowsi university of Mashhad 2. University of Medical Sciences of Mashhad
Abstract
Introduction
Although variola and vaccinia viruses have been eradicated but still smallpox can be a serious problem for societies. h3l is a conserve protein among agent of smallpox viruses and is highly immunostimulant.
the aim of this study first is bioinformatics analysis, epitope prediction and modeling of h3l epitopes and the second aim is validating and molecular docking of an experimental epitope in h3l against mhcⅠ receptors.
Methods
In smallpox since cd4-t has the most important rule in development of immune response, epitopes of h3l have been predicted for mhcⅠⅠ receptors based on the most frequency, highest score and sharing between servers with different algorithms by bioinformatics servers. the potency of the epitopes was investigated by vaxijen 2.0 with 0.6 threshold. second and third structure of mhcⅠ receptors and experimental epitope downloaded from rcsb database as a crystallography pdb file. models of predicted epitopes that were absent in crystallography of h3l were created by i-tasser.
Results
The results of this research were 5 epitopes for 6 mhcⅠⅠ receptors. molecular docking for mhcⅠ receptor and experimental epitope demonstrate powerful connection.
Conclusion
In future using epitopes of h3l can assist to produce recombinant vaccine against smallpox and reduce the risk of using traditional vaccines.
