Resistance to antibiotic treatments is one of the increasing problems. therefore, alternative methods to reduce antibiotic resistance are essential. today, antibiotic resistance is growing in the causes of infectious diseases such as salmonellosis. the use of bacteriophages, or in short, phages (viruses that attack bacteria and eliminate them) is one of the new approaches in this field.
Methods
Standard strains of salmonella (enteritidis, typhimurium and infantis) were extracted from the faculty of veterinary medicine, university of tehran, and their specific bacteriophages were isolated using soft agar method. in the next step, the direct effect of each strain on the target cell line was investigated, and the effect of the inoculated bacteriophage on each of the strains was measured.
Results
Particular plaques of bacteriophages isolated from salmonella were specific to each salmonella, and had no lytic performance against other salmonella and other intestinal pathogenic bacteria. the binding and pathogenicity of salmonella strains were evaluated positively as expected, and after inoculation of specific bacteriophages, their lytic function was well observed.
Conclusion
Overall, given the increasing prevalence of antibiotic resistance among salmonella and the concern about treatment, in the future, these phages can be well used for disease control and prevention, both in vivo and in vitro conditions.
