Frequency of tem and shv genes in the strains of acinetobacter producing broad-spectrum isolated ß-lactamases from zahedan patients by pcr method

Somayeh Iranian,1,* Mahshid amir mohammadi dowlat abad,2 Iman dehbashi,3 Samira irani,4

1. Experimental sciences scientist, Islamic Azad University, Zahedan Branch, Iran.
2. Graduate Student of Nursing, Department of Nursing, Faculty of Medicine, Zahedan Islamic Azad University, Iran.
3. Graduate Student of Nursing, Department of Nursing, Faculty of Medicine, Zahedan Islamic Azad University, Iran.
4. Expert in Physical Education, Islamic Azad University, Zahedan Branch, Iran.

Abstract

Introduction

Acinetobacter baumannii is one of the most important factors in hospital infections, and its colonization rate in hospitalized patients, especially long-term patients, or those treated with broad-spectrum antibiotics or anticancer drugs. it's rising. antibiotic resistance is now increasing in acinetobacter baumannii and this is an important factor in colonization and infection in patients, especially patients admitted to the icu. the purpose of this study was to identify esbl genes in acinetobacter baumannii strains bacteria resistant to third generation cephalosporins are clinical specimens by pcr.

Methods

This cross-sectional study was performed on 86 actin bacteria isolated from patients in zahedan during 1994-1998. differential and biochemical tests were performed to identify the isolates and then antibiotic resistance patterns were determined by disc diffusion method. combined disk method was used to confirm the production of esbl in western organisms. by pcr, tem and shv genes were detected in these isolates.

Results

The highest sensitivity of the bacteria to the antibiotics was amikacin (29.06%), imipenem (13.95%) and gentamicin (9.3%) respectively. also, some isolates were semi-sensitive as against the antibiotics. the bacteria showed high resistance to cefotaxime antibiotics, ceftazidime, ceftriaxone, aztreonam and ciprofloxacin. pcr results showed that all isolates were negative for shv gene. while 87.02% of the tem gene was positive.

Conclusion

Resistance to many antibiotics in the baconian acinetobacter baumannii isolates is increasing and the tem gene plays an important role in the resistance to third-generation cephalosporins in this bacterium.

Keywords

Acinetobacter, antibiotic resistance, tem and shv genes, broad-spectrum beta-lactamases.