Frequency and pattern of resistance of ampc-beta-lactamase gene in clinical isolates of klebsiella pneumoniae from zahedan educational hospitals

Mahshid Amir mohammadi dowlat abad,1,* Somayeh irani,2 Iman dehbashi,3 Zoharayeshi zeidabad ,4 Faezeh sarahidi,5

1. undergraduate nursing student, Department of Nursing, Faculty of Medicine, Zahedan Islamic Azad University
2. Bachelor of Science, Islamic Azad University, Zahedan Branch
3. undergraduate nursing student, Department of Nursing, Faculty of Medicine, Zahedan Islamic Azad University
4. Undergraduate Student, Midwifery Department, Faculty of Medicine, Zahedan Islamic Azad University
5. undergraduate nursing student, Department of Nursing, Faculty of Medicine, Zahedan Islamic Azad University

Abstract

Introduction

The detection of ctx-m-15 genes in esbls produced by klebsiella pneumoniae strains provides useful information on epidemiology and treatment and the risk factors involved in these infections. antibiotic resistance in klebsiella pneumoniae is one of the causes of hospital infections. the aim of this study was to determine the presence and abundance of ctx-m and ctx-m-15 genes and to determine the antibiotic resistance of cases of clinical infections in zahedan.

Methods

This cross-sectional study was performed on khatam, nabi-akram and bou-ali hospital in zahedan. 132 clinical samples were collected and cultured for isolation of klebsiella pneumoniae. control strains, dna were extracted by lysis method and used for pcr test to identify the genes.

Results

Of the samples, 88 strains of klebsiella pneumoniae were isolated, of which 20 strains had at least one of the examined genes. of the 20 positive strains, 19 strains (20.65%) had the ctx-m-15 gene and one strain (1.08%) containing both ctx-m and ctx-m-15 genes. the highest and the lowest antibiotic resistance was amoxicillin 85 strains (92.39%) and piperacillin 37 strains (40.21%) respectively.

Conclusion

Antibiotic resistant strains can be prevented by identifying antibiotic resistance patterns and beta-lactamase.

Keywords

: klebsiella pneumoniae, ampc gene, antibiotic resistance.