Genes involved into both alternative splicing and aging pathways as biomarkers for parkinson disease diagnosis

Soudabe Fazeli,1,* Kamran ghaedi,2 Maryam peymani fouroushani,3

1. Biology department, Faculty of Science, University of Isfahan, Isfahan
2. Cellular Biotechnology department, Research Center for Cellular Sciences Jahad University ,Royan Biotechnology Institute
3. Biology department, Faculty of Basic Science, Islamic Azad University of Shahrekord Branch, Shahrekord

Abstract

Introduction

parkinson's disease is a degenerative movement disorder that exhibit symptoms through progressive loss of dopaminergic neurons into substantia nigra. referring to our studies based on two relational loop between pd, splicing and ageing, and also relation these two recent together, and correlation epigenetic (one of the aging acceleration biomarkers) into blood with pd, using blood transcriptomic of patients with early pd, we provided molecular insight for dysfunction alternative splicing and ageing pathways in pd.

Methods

We selected four studies from blood transcriptomes of drug naïve patients and early pd using geo datasets. we selected genes with differential expression. using gene ontology and molecular signatures databases, signaling pathways were determined for genes with differential expression in these four microarrays. eventually, genomic network analysis was performed through genemania software.

Results

We performed a meta-analysis using nextbio for dissection this whether differential expression into blood can provide knowledge about alternative splicing and aging disturbed pathways in pd. srrm2 gene is one of the splicing factors involved in splicing signaling and it was downregulated in two transcriptomic arrays. srpk1 gene like the previous gene, this is involved in splicing signaling and it revealed downregulation. lmna gene is among genes very close to srrm2 and its protein include lamin family and this gene is one of the upstream genes aging caused by accumulation of defective dna repair.

Conclusion

Genes considered in this study can present as biomarkers for pd diagnosis, because blood selective tissue is a less invasive tissue and also these selected from early pd and drug naïve patients. high-throughput screening of blood rna have provided molecular clues for some of the dysregulated pathways in pd, including the impairment of insulin signaling and glucose metabolism, aberrant rna splicing and inflammation

Keywords

Parkinson disease, splicing, aging, biomarker, diagnosis.