1. Department of Biology, Faculty of Sciences, Urmia University
Abstract
Introduction
Minoxidil is the most common drug used for treatment androgenic alopecia.studies have shown that this drug can increase the length of hair growth phase (anagen) and early transfer of hair follicle from catagen phase to anagen.studies on the mechanism of minoxidil are rare and still target cells and signaling pathway that minoxidil because it can exert its effect,is not kown.in this study we in vestigated and evaluated the expressin of akt1 , erk1 , kras1 , shh , bmp4 , b-catenin ,usind real time-pcr.bulge and bulb region cells cultured and treated with different concentrations of minoxidil , so cell survival were evaluated by mtt.
Methods
The mouse hair follicles were isolated on day 10 after
depilation and bulge or dp regions were dissected. the bulge and dp cells were cultured for 14 days in
dmem/f12 medium. then, the cells were treated with 100mm minoxidil for 10 days.
nuclear morphology was identified using dapi staining. reverse transcriptase and real-time polymerase
chain reaction (pcr) analysis were also performed to examine the expression of kras, erk, akt, shh and
b-catenin mrna levels in the treated bulge and dp regions after organ culture.
Results
The result of mtt and acridin orang-ethydium bromide staining showed the level of gene expression were evaluated by real time-pcr.our results showed that expression of shh and erk1 after treatment with minoxidil asignificantly increased compared the control group.that high concentrations of 500 μg minoxidil increased cell deth and necrotic
Conclusion
The finding of this study showed the minoxidil increase accelerating the onset of anagen shh gene expression of certain genes and erk1 and kras1confirm the fact that minoxidil through the kras-raf-erk signaling enhances cell proliferation and apoptosis is protected.
Keywords
Bulge region ; hair follicle stem cells; bulb cells; all trans retinoic acid; minoxidil
