cancer is a fatal disease caused mainly by environmental factors that mutate genes encoding critical cell-regulatory proteins. nf-kappab induces the expression of diverse target genes that promote cell proliferation, regulate apoptosis and stimulate invasion and metastasis.traf1 and traf2 were identified as gene targets of nf-κb transcriptional activity. in the present study, we evaluated promoter methylation changes at nfk-b ،traf1 and traf2 genes in oscc pationts and normal controls.
Methods
Paraffin was-embedded tissue samples of oscc (n=86) and normal oral mucosa (n=68) were collected from individuals referred to the dental school at zahedan university of medical sciences. dna was isolated from tumor and healthy tissue samples using qiaamp dna extraction kit. dna methylation patterns in the cpg islands were determined by chemical treatment with sodium bisulfite and subsequent methylation specific pcr(msp) using methylated specific and unmethylated specific primers. data were analyzed using chi-square test in spss software.
Results
Analysis of data showed that promoter methylation at nfk-b was significantly different in cases compared to healthy controls (p = 0.00), but no difference was detected for the traf1 and traf2 genes (p <0.843 and p <0.253 respectively)..
Conclusion
Due to the decreased methylation of the promoter of the nfk-b this gene plays an important role in the development of oral cancer, and it seems to be used as a biomarker for diagnosis.
