Synthesis and characterization of gold nanoparticles by isolated bacterial from kerman sarcheshmeh copper mine soil and the study of their cytotoxic properties

Mandana Jafari,1,* Hamid frootanfar,2 Farokh rokhbakhsh zamin,3 Atefeh ameri,4

1. Pharmaceutics research center, institute of neuropharmacology, Kerman University of medical science, Kerman, Iran
2. Pharmaceutics research center, institute of neuropharmacology, Kerman University of medical science, Kerman, Iran
3. Department of Microbiology, Faculty of Sciences, Islamic Azad University, Kerman Branch
4. Pharmaceutics research center, institute of neuropharmacology, Kerman University of medical science, Kerman, Iran

Abstract

Introduction

The present study was designed to isolate bacterial strain capable of gold nanoparticles (au nps) production followed by purification and evaluation of the cytotoxic effect of aunps.

Methods

Among several environmental samples collected for screening of au nps -producer bacterial strains one bacterial colony (isolated from kerman sarcheshmeh copper mine soil as pseudomonas pseudoalcaligenes strain) was selected and applied for biosynthesis of au nps. the cytotoxic effect of biologically synthesized au nps and au3+ on two cell lines of mcf-7and a549 was then determined using the mtt assay method.

Results

The isolated bacterial strain is belongs to pseudomonas family and it was identified as pseudomonas pseudoalcaligenes. the obtained results revealed lower toxicity for the biogenic gold nanostructures compared to au3+.

Conclusion

Chemical methods are quite energy-intensive and generate environmentally hazardous waste owing to using chemicals. physical methods are quite time-consuming. owing to the growing application of gold nanoparticles, there is a need for mass production of these nanoparticles by biological systems. different microorganisms are used to synthesize gold nanoparticles including prokaryotic organisms such as acinetobacter, pseudomonas aeruginosa and eukaryotic organisms such as the fungus fusarium oxysporum as a green bio factor. gold nanoparticles are widely used in medicine for diagnosis and treatment of cancers.

Keywords

Biological synthesis, gold nanoparticles, cytotoxic, pseudomonas pseudoalcaligenes