1. Department of Biomedical Engineering, Islamic Azad University, Tehran Markaz-Branch 2. Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University 3. Assistant Professor, Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University
Abstract
Introduction
Doxycycline (doxy) is a second generation tetracycline antibiotic with extensive usage. high concentration of doxy might be toxic to healthy tissues and prolonged use of this drug may cause superinfection. modern drug delivery systems play an important role by eliminating the side effects of frequent dosing. nano carriers such as nanofibers have been receiving special attention to minimize drug side effects, usage doses and associated toxicity. we incorporated doxycycline into kefiran (kef) nanofibers and obtained improvement in cell viability compared to free drug
Methods
Kef extracted from kefir grains was used as a carrier to reduce cell toxicity of doxy. drug with highest concentration possible was used (1200 μg /ml) in order to entrap within nanofibers without bead formation. the mtt assay was performed on doxy, drug loaded nanofibers, and kef to evaluate their cell toxicity. mats were assessed on l929 fibroblast cell line. the surviving cell percentage after 24h and 72h was reported
Results
Mtt assay revealed that 67% of cells survived in the presence of doxy loaded nanofibers after 24h. compare to cell viability results for doxy with 1200 μg /ml concentration, there is 41% increase in cell viability. in addition, because of drug toxicity, cell viability in doxy loaded nanofibers decreased to 50% after 72h. also, kef nanofibers showed 70% cell viability in 72h
Conclusion
Overall, mtt assay showed increasing cell viability of drug loaded nanofibers in compare to drug itself. kef nanofibers could have a great potential to reduce side effects of antibiotics for drug delivery applications
