1. tarbiat modares university 2. tarbiat modares university 3. Iran Polymer and Petrochemical Institute
Abstract
Introduction
Recently, some novel drug delivery systems were designed to control drug release. the using of polymeric nanoparticles as the carrier is one of this systems. in this study, chitosan was used as a carrier for vancomycin. preparation of nanoparticles and optimization of parameters that affected on loading content were investigated.
Methods
Low molecular weight of chitosan, tripolyphosphate(tpp) and acetic acid were obtained from sigma and vancomycin was purchased from danapharma company. chitosan nanoparticles were prepared by using ionic gelation method. the effect of chitosan solution content, the ratio of chitosan to tpp and drug level were investigated for three effective parameters in drug loading and particle size. for this purpose, box-behnken; a statistical method was used to design experiments in three levels.
Results
The nanoparticles were optimally spherical and had a surface charge of +35mv. the average size of particles without drug and the particles containing drug were measured 130nm and 174 nm respectively, which this difference was caused by loading of the drug. drug loading efficiency was 40% and drug loading content was 67%. 32% of drug that was loaded, released at first 24 hours and 70% of drug loaded released in 20 days in buffer acetate at ph 6.9.
Conclusion
Loading vancomycin in chitosan nanoparticle facility controlled and sustained drug release for infectious diseases such as osteomyelitis.
