1. Department of genetics,tonekabon branch,islamic azad univercity,tonekabon,iran 2. Department of microbiology,tonekabon branch,islamic azad univercity,tonekabon,iran
Abstract
Introduction
It has been proven that cancer cells formation can be due to the herpes simplex virus infection in human beings. the aim of this study is to assess the quantity of herpes simplex virus’s dna in the fresh and paraffin fixed samples of human breast tumors.
Methods
70 fresh and paraffin fixed samples of human breast tumors were collected from several hospitals (city of rasht, north of iran). dna extraction was carried out via genall korea kit. special primers were used for amplification of the ul30 gene of herpes simplex virus.
Results
Based on the demographic data, the average age of most patients was between 40 to 50 (41.43%), and less than 10% of them were younger than 40 years old. the results showed no positive cases among all the 70 samples, exhibiting no herpes simplex virus genome in breast tumor.
Conclusion
The interference of herpes viruses in cervical cancers, hodgkin's and burkitt's lymphoma and cancer of blood vessels has been proven for a decade. the results of some studies, however, reported no hsv genomes in tumors. more assessments therefore are needed for understanding the association between these types of viruses, and breast cancer.
