Investigation of toxicity effect of 4-mepgc and 4-no2pgc on the k562 cell line (chronic myeloid leukaemia)

Fateme Asgari,1,* Roya mahinpur,2 Nooshin haghighipur,3 Lila moradi,4

1. Department of Biotechnology, University of Kashan, Kashan, Iran
2. Department of Organic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran
3. National Cell Bank of Iran, Pasteur Institute of Iran,
4. Department of Organic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran

Abstract

Introduction

Leukemia is a particular type of cancer characterized by the failure of cell death or disability in the differentiation of hematopoietic cells. chronic myelogenous leukemia (cml) is the most studied type of this cancer. chromene (benzopyran) is one of the privileged medicinal pharmacophores which appears as an important structural component in natural compounds and generated great attention because of their interesting biological activity. it is a heterocyclic ring system consisting of a benzene ring fused to a pyran ring. it is known that certain natural and synthetic chromene derivatives possess important biological activities such as antitumor, antivascular2, antimicrobial3, antioxidant4 activity, therefore, the aim of this study is to investigate anti-cancer effect of some 4-mepgc and 4-no2pgc on the k562 cell line on the human leukemia k562 cells

Methods

The human k562 cell lines were obtained from the cell bank at the pasteur institute of iran and were cultured in rpmi-1640 medium supplemented with 10% fetal bovine serum, penicillin (100 u/ml) and streptomycin (100 µg/ml) at 37ºc and 5% co2 and then treated with different concentrations of the investigated dihydro-pyranochromenes and cell viability was assessed using the mtt assay and confirm the results of the previous analysis, also, apoptotic cells were quantified by annexin v/pi double staining method with analyzing of phosphatidyl serine on the outer surface of apoptotic cell membranes

Results

Among two compounds, 4-no2pgc was determined as a stronger compound with an ic50 value of 118±2.14 in 48 h. all of this compounds have little toxicity in pbmc. according to results of flow cytometry, the most induction of apoptosis was 4-no2pgc

Conclusion

Our results also confirmed that these compounds may be provide a new therapeutic approach for the treatment of leukemia.

Keywords

chroneme compounds; k562 cell : mtt: flow cytometry;