Astaxanthin decreases cell viability in mda-mb-231, but not in non-tumorigenic human breast cell line mcf 10a
Aida Karimian,
1,* Mir mohammadrezaei fereshteh,
2 Bahadori mohammad hadi,
3 Hajizadeh moghadam akbar,
4
1. Faculty of Science, Student Of Mazandaran University, Babolsar, Iran
2. Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
3. 3. Cellular and molecular research center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
4. 4. Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Abstract
Introduction
Considering the high prevalence of breast cancer in iran, its rising incidence, and the high mortality rate of patients afflicted with it, we decided to study the effects of astaxanthin on breast cancer cells including mda-mb-231 and non-tumorigenic mcf 10a cell lines.
Methods
Mda-mb-231 cancer cells and non-tumorigenic mcf 10a cells were cultured in dmem and mebm culture media, respectively. after selecting effective astaxanthin doses and treating cells of both cell lines with them, the effects of the doses on cell viability after various lengths of time were studied using the mtt assay. cell apoptosis was studied employing acridine orange/ethidium bromide (ao/eb) staining. all data were statistically analyzed using the one-way anova, graphpad prism software (p < 0.05).
Results
Our findings indicated that astaxanthin has cytotoxic effects on mda-mb-231 but not on mcf10a. mtt assay results revealed that ic50 values of astaxanthin on mda-mb-231 cells were 65.383±4.597 and 20.786±7.580 µl after 24, 48 h. mentioned doses have shown no significant changes in cell viability of non-tumorigenic human breast cell line (mcf 10a).
Conclusion
Altogether, our study has shown that changes in the level of cellular toxicity in mda-mb-231 cell lines might reveal the efficacy of astaxanthin. further studies with other tests are helpful to confirm these effects.
Keywords
Astaxanthin, mda-mb-231, mcf 10a, cell viability
