An investigation on the structure and function of the cell surface lung-specific antigens for development a new generation of immunotoxins

Bahareh Hasani sabzevar,1,* Aliakbar haddad- mashhadrizeh,2 Jafar saeidi,3

1. Department of Biology, Islamic Azad University, Khorasan Razavi, Neyshabur, Iran
2. Cell and molecular biotechnology research group, institute of biotechnology, and Department of Biology, Faculty of Scien
3. Department of Biology, Islamic Azad University, Khorasan Razavi, Neyshabur, Iran

Abstract

Introduction

Lung cancer, with common symptoms such as coughing, weight loss and shortness of breath, is a disease via uncontrolled growth of the cells in the lung tissues and such as other cancers is lethal if postponed its diagnose and treatment

Methods

In this regard, several therapeutic and diagnostic procedures have been developed, which among of them immunotoxins with target therapy capacity have promising vision. bearing in mind, in order to designing and optimization a new version of specific immunotoxin for targeting lung cancerous cells and diagnostic method, in-silico expression of the cell surface lung-specific antigens (lsas) were assessment and corresponding ligands were detected. at the first step, lsas were investigated via literature review. in-silico expression of the selected antigens were performed via proteinatlas program, on the several of cancerous and non-cancerous tissues, and evaluated via spss analysis. on the other hand, string and haddock2.2 webservers were using for detected corresponding antigens and evaluated their binding affinity, respectively

Results

Our investigation led to gathering 20 lsas, based on literature. ceacam8, ceacam6, epcam are selected with high expression on the surface of lung cancerous cells as candidate for immunotoxin development and diagnostic procedure. on the other hand, 3d structure of selected antigens were determined with high quality in errat and rampage plots

Conclusion

Finally, cam6 are selected as corresponding ligands of these antigen with high and specific affinity

Keywords

Lung cancer, immunotoxins, antigen, ligand