1. Cellular ana Molecular Research Center, Basic Health Sciences Institiute, Shahrekord University of Medical Sciences 2. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences 3. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences
Abstract
Introduction
Influenza a virus (iav) can cause acute respiratory distress syndrome, which leading to kill 250000 to 500000 of world population annually. studies show that the complications of severe influenza infection and death in addition to viral replication are due to overproduction of pro-inflammatory cytokines induced by the virus. current antiviral drugs for the treatment of iav infection do not target mechanisms that result in overproduction of the pro-inflammatory cytokines. therefore, a new compound needs to use with current antiviral drugs to reduce overproduction of pro-inflammatory cytokines in iav infection. in this study, the effect of celastrol as a herbal and anti-inflammatory compound was investigated on tumor necrosis factor alpha (tnf-α) gene expression levels that are induced by iav (h1n1; pr8) in madian-darby canine kidney (mdck) cells.
Methods
Confluent mdck cells were infected with 100tcid50 of iav and treated with different concentrations of celastrol. after 24 h incubation, the cells were collected for measuring of tnf-α gene expression compared to virus control by real-time pcr according to the manufacturer's protocol.
Results
Gene expression levels of tnfα increased significantly in virus control compared to cell control, and decreased significantly compared to virus control after treatment with celastrol at different concentrations (p < 0.05).
Conclusion
Tnf-α is a pro-inflammatory cytokine that plays an important role in inflammation, lung injury, severity of disease symptoms and death. therefore, celastrol with reduction of tnf-α gene expression can be a suitable option to control of inflammation from cytokines in iav infection, so it can be used with current antiviral drugs.
