1. Alborz University of Medical Sciences 2. Alborz University of Medical Sciences
Abstract
Introduction
One of the most common bone metabolic and endocrinal disorders is osteoporosis. it is defined by progressive bone loss, deterioration of microscopic structure of bone tissue and loss bone marrow. it leads to bone fractures especially in women and its mortality is equal to cancer breast! in this study we used zinc (zn) trace element and vitamin e (vit e) as an antioxidant to evaluate bone healing parameters.
Methods
40 three month old female wistar rats were selected. randomly (n = 7) as control and the others were ovariectomized and divided into 4 groups: sham (n = 7), treatment with vit e (n = 9) 200iu/day, with zn (n = 9) (200mg / lit) and with zn + vit e (n = 8). after 8 weeks, animals were sacrificed and blood sample and right femur was taken. bone sections was stained with h & e and von kossa staining. furthermore electron microscopic (em) study was done.
Results
Osteoid tissue was better formed in compacted eosinophilic areas in vit e+zn groups. non treated rats had a significant decreased bone volume and number of trabeculae as to baseline. calcitonin and zn levels in blood serum in groups treated with zn + vit e was significant comparing to other groups. the em graphs showed osteoblast cells with wide cytoplasmic processes in experimental groups. the large and irregular surface of cells was indicate intense activity. abundant deposits crystals and particles of calcium were visible appearance of collagen fibers visible and connective tissue and collagen fibers increase was seen in all areas.
Conclusion
Our result may lead to a new sight to treatment of osteoporosis by activation of re absorbing in bone tissue. increasing of osteoblast cellular activity and bone mass formation, indicate important role of co-treatment of zn and vit e to prevent bone loss in reducing bone loss and increasing of calcitonin as an index to reduce osteoporosis.
