An investigation on the structure and function of the cell surface breast-specific antigens for development a new generation of immunotoxins

Fereshteh Ghaderi,1,* Aliakbar haddad- mashhadrizeh,2

1. Pharmaceutical Science Group , Khayyam Bioeconomy Institute (KBI), Mashhad, Iran
2. Cell and molecular biotechnology research group, institute of biotechnology, and Department of Biology, Faculty of Scien

Abstract

Introduction

Breast cancer with heterogeneous features in morphology, clinic, and responses to therapeutic options, is one of the most common cancer in the world which originated from adenocarcinoma tissues. bearing in mind, there are expanded diagnostic and therapeutic strategies in association with this type of disease based on cell surface specific antigens such as immunotoxins drugs. accordingly, in order to designing and optimization a novel immunotoxin for targeting breast cancerous cells, as well as developing diagnostic method, in-silico expression of the cell surface breast-specific antigens (bsas) were assessment. moreover, corresponding ligands of these antigens were detected

Methods

, bsas were investigated via literature review. in-silico expression of the selected antigens were performed via protein atlas program, on the several of cancerous and non-cancerous tissues, and evaluated via spss analysis. on the other hand, string and haddock2.2 web servers were using for detected corresponding antigens and evaluated their binding affinity, respectively

Results

Our investigation led to gathering 13 bsas, based on literature. ceacam5, ceacam6, tpm1 are selected with high expression on the surface of breast cancerous cells as candidate for immunotoxin development and diagnostic procedure. on the other hand, 3d structures of these antigens were determined with high quality in errat and rampage plots.

Conclusion

Ceacam8 is selected as the best corresponding ligand of ceacam6 antigen with high and specific affinity.

Keywords

Breast cancer, immunotoxins, antigen, ligand