1. Islamic Azad University, Zanjan Branch, Faculty of Baisic sciences, Department of Genetics, Etemadeyeh, Moalem St. 2. Islamic Azad University, Zanjan Branch, Faculty of Baisic sciences, Department of Genetics, Etemadeyeh, Moalem St.
Abstract
Introduction
The study of her-2 gene in cancers, especially gastric cancer, is of great importance. this gene, has been shown to increased expression rate in gastric cancer. the aim of this study was to investigate level of her-2 gene expression in gastric adenocarcinoma cells after treatment of the cells with iranian saffron extract. the advantage of this extract is its anti-cancer properties and the absence of side effects.
Methods
105 ags cell were cultured in 12 wells plate. the cells were treated with concentrations of 800, 1200, 2000 μg / ml of iranian saffron extract for 48 hours and then rna extraction and cdna synthesis was performed using special kits. finally, the her-2 expression rate examined with specific primers using real time pcr. gapdh gene was used as an internal control. data analysis was performed with 2 - ΔΔct .
Results
The results of real time pcr data have shown in 48 hours treament, reduction of 2 fold in her_2gene experession with cincentration of 2000μg/ml but in concentration of800and1200μg/ml there was no significant reduction of her-2 expression.it seems that the effect of this extract is in higher concentrations and also it need to examine more concentrations.
Conclusion
Herbal extract seem to be a good alternative to chemical drugs, given the effectiveness of preventing tumor growth and less side effects.
