Evaluation of interleukin 10 gene promoter polymorphisms of -592a/c and -1082a/g region among obese individuals with type 2 diabetes in alborz province

Farzin Ferdousi,1,* Massoud houshmand,2 Mahdi nazari,3 Samaneh layeghi,4

1. Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2. Department of Medical Biotechnology, National institute of genetic Engineering and Biotechnology
3. Department of Genetic,Biology Research Center,Zanjan Branch,Islamic Azad University,Zanjan,Iran
4. Department of Genetic,Biology Research Center,Zanjan Branch,Islamic Azad University,Zanjan,Iran

Abstract

Introduction

Today diabetes is one of the most prevalent noncontagious diseases around the world. there is strong evidence showing inflammatory reactions play a crucial role in initiating this disease. overweight and obesity are common risk factors for type 2 diabetes (t2d) and most of the time body composition controlled by genetic factors. interleukin 10 is known as one of the most important anti-inflammatory cytokine. . thus, the present study was conducted to investigate the relationship between interleukin 10 gene polymorphisms -592 a/c and -1082 a/g and susceptibility to get t2d and obesity

Methods

In this study, 50 obese patients with t2d, 50 non-obese patients with t2d, 50 obese patients without t2d and 50 healthy subjects were selected. dna extraction was carried out and then the genotype in the -592 a/c and -1082a/g positions were determined using pcr-rflp while chi squared (x2) was used as the statistical method for data analysis

Results

The frequency of cc and ac genotype in -592a/c position in obese patients with t2d and non-obese patients with t2d had more importance than non-diabetic patients (p<0.05). also the frequency of aa genotype in -1082a/g position in obese patients with t2d was significantly more than other subjects (p<0.05)

Conclusion

The high frequency of c allele in -592a/c can be considered as a predisposing factor for type 2 diabetes. understanding the basis of this heterogeneity provides an opportunity for personalizing prevention and treatment strategies according to individual patient clinical and molecular characteristics

Keywords

Type 2 diabetes, obesity, polymorphism, interleukin10