Thiosemicarbazone compounds have previously been considered as a new series of potent apoptosis-inducing agents. in the present study, we evaluated the influence of an active compound from methyl-thiosemicarbazone family on growth inhibition and induction of apoptosis in k562, chronic myeloid leukemia cells
Methods
The viability and growth inhibition of the treated cells were measured in various concentrations (10-120 μm) during times 24, 48 and 72 hours by mtt assay. results showed that the viability and proliferation decrease dose dependently at during 24-72 hrs. the ic50 value was determined as 80±3.0 μm. analysis by flow cytometry revealed sub-g1 cell cycle arrest in k562 cells treated with this compound
Results
Results showed that the viability and proliferation decrease dose dependently at during 24-72 hrs. the ic50 value was determined as 80±3.0 μm
Conclusion
Based on these data, it seems that methyl-thiosemicarbazone complex with cu2+can be considered as a good candidate for next pharmaceutical evaluations
