as the antibiotics are misused, integrons are the moving genetic factors which carry resistance gens and have an important role in spreading drug resistance among the enterobacteriaceae family. the end point of this study is to evaluate the role of class 1 and 2 integrons in forming drug resistance in uropathogenic escherichia coli strains taken from urine samples of patients in tehran’s hospitals
Methods
we studied 60 different strains of escherichia coli, all taken from urine samples of patients in tehran city’s hospitals. the bacteria were identified using standard biochemical tests and their antibiotic sensitivity pattern to 9 antibiotics was specified through disk diffusion method. the intended strains were studied for presence of class 1 and 2 integrons using primers allocated to target class 1 and 2 integron gens and through pcr method.
Results
of 60 e.coli 36 (60%) strain were class 1 integron. class 2 integron was detected in 3 (5%) strains and 95% isolates were multi-resistant to antibiotic. antibiotic susceptibility results stated that chloromanphenicol has the lowest resistance vate (10%) and the greatest resistance was correspondant to streptomycin, (90%) respectively. a direct correlation was revealed between class 1 integron and resistance to streptomycin, nalidixic asid, norfloxacin and ampicillin, and also between chass 2 integron and resistance to, streptomycin , nalidixic asid, cefotaxime and ceftriaxone.
Conclusion
Our results show that the prevalence of class 1 integrons is higher comparing to class 2 and this will cause the multi-drug resistant strains to spread and makes the treatment of infections more complicated in future
