Effect of marjoram extract on sperm concentration and its survival percentage in oxidative-cadmium stress in adult male rats

Azad Bahrampour,1 Mehdie raeeszadeh ,2,* Mohamad mehdi fallah,3

1. Student of veterinary Medicine , Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2. 1Assistant professor in Pharmacology, Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj
3. D.V.M, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Abstract

Introduction

Nutrition safety and health is one of the important factors in fertility.cadmium as an environmental pollutant by free radical production can damage the sperm and thus reduce fertility. the aim of this study was to evaluate the effect of marjoram extract on sperm concentration and its survival rate in oxidative cadmium injury in rats.

Methods

An experimental- interventional study was performed on 24 male wistar rats weighing 200-220 grams. animals were randomly divided into 4 groups of 6. the control group received cadmium chloride at a dose of 2 mg / kg by intraperitoneal method then test group include t1, t2 and t3, in addition to the cadmium, received marijuana aqueous extract and received doses of 100, 200 and 400 mg / kg for 10 days, respectively. on the 11th day, animals were weighed. blood samples were taken from their heart to measure the total antioxidant capacity of the serum. animals were then exterminated humanely. right testis was used to measure the concentration of malondialdehyde and its tail epididymis for sperm counting and sperm survival.

Results

At the end of the study, the lowest weight of animals in the control group was 195.66 grams, which showed a significant difference with other groups. in association with malondialdehyde, the highest concentration was observed in the group receiving cadmium chloride with a mean of 1.4 μmol / ml and the lowest in t3 group with 0.93 μmol / ml. there was a significant difference between the control group and the rest of the experimental groups .total serum antioxidant concentrations in t3 group had the highest concentration of 1198.191 μmol / ml, which had a significant difference with control group with a concentration of 796.4 μmol / ml. the highest number of sperm in the t1 group was 106× 66/21 the percentage of membrane health in the t1 and t2 groups was approximately 33% and 34%, the t3 group was 11% and the control group was approximately 6%. this difference was significant between the control and other groups. the results show that in the 400 mg / kg extract, the membrane's health is reduced.

Conclusion

The phenolic compounds and flavonoids in the marjoram extract are responsible for the antioxidant effects of the extract, which in the desired dose has an effective effect on controlling the effects of cadmium on sperm.

Keywords

Infertility, oxidative stress, cadmium chloride, marjoram aqueous extract, male rats