The Number of Registered Candidates in Congress: 4667 People
Frequency of escherichia coli o157:h7 among children with and without diarrhea stool samples referred to microbiology laboratory of shahid madani hospital in khorramabad during 2016-2017.
1. LorestanUniversity 2. LorestanUniversity 3. Lorestan University Medical Sciences 4. Lorestan University Medical Sciences
Abstract
Introduction
Escherichia coli serotype o157:h7, which has been recognized as a food-borne pathogen occurring worldwide in the recent decade, is associated with a large spectrum of illness including
bloody or non-bloody diarrhea, hemorrhagic colitis and haemolytic-uraemic syndrome (hus). the aim of this study was to survey the prevalence of e. coli o157:h7 in diarrheal stool samples of children under the age of ten were referred to microbiology laboratory of shahid madani hospital in khorramabad city.
Methods
In this cross sectional study, 400 stool samples (200 diarrheal and 200 non-diarrheal) were screened for the presence of e. coli o157:h7 using culture and multiplex pcr. samples were cultured onto selective solid media namely sorbitol macconkey agar supplemented with cefixime and tellurite (ct-smac) by streaking and incubated at 37°c for 18-24hours.the colonies that appeared colorless on ct-smac were tagged as presumptive e. coli o157 and were serologically confirmed as e. coli o157:h7 using the latex agglutination
test kit and multiplex pcr.
Results
Out of 400 stool samples tested, only 46 cases (11.5%) were sorbitol-negative. none of the sorbitol negative e. coli colonies were not confirmed by agglutination with e. coli o157:h7 antiserum and multiplex pcr using specific primers.
Conclusion
Although e. coli o157:h7 is not the major cause of pediatric infections in this region of iran, further studies with greater sample size are needed to confirm our results.
