The effect of mouse recombinant resistin on blood- brain barrier permeability, mda content and apoptosis in the early stages of focal cerebral ischemia in a stroke mouse model

Sedigheh Behrouzifar,1,* Abedin vakili,2 Ahmadreza bandegi,3

1. Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
2. Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
3. Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.

Abstract

Introduction

The aim of this study was to evaluate the dose-dependent effects of mouse recombinant resistin on blood-brain barrier(bbb) permeability, mda content and apoptosis in a mouse model of stroke.

Methods

Transient focal cerebral ischemia was produced by the middle cerebral artery occlusion (mcao) in mice. resistin at doses of 25, 50, 100, 200, and 400 ng/mouse, was administered intracerebroventricularly (icv) at the beginning of mcao. at 24 h after inducing the ischemia, blood- brain barrier permeability, mda content and apoptosis were assessed by using the standard methods. bbb disruption was determined by quantifying the eb leakage into ischemic brain tissue. the malondialdehyde (mda) content was measured using the thiobarbituric acid method. tunel staining was used in order to assess the number of apoptotic cells in three different regions of right brain hemisphere.

Results

Resistin at the most effective dose (400 ng/mouse) significantly decreased evans blue (eb) leakage (p < 0.05), mda content (p < 0.005) and apoptosis (p < 0.001) at 24 h after ischemia.

Conclusion

Our findings showed that treatment with the mouse recombinant resistin could ameliorate bbb disruption, apoptosis and oxidative stress in a dose-dependent approach in the early stages of focal cerebral ischemia. further investigations are recommended to clarify the molecular mechanisms of function of resistin on the brain ischemia and apply it as a therapeutic target for the stroke patients.

Keywords

recombinant resistin; cerebral ischemia; blood-brain barrier permeability; mda content; apoptosis