Higher efficacious delivery in non-toxic dose of genistein to aml cells by its conjugation on superparamagnetic fe3o4 nanoparticles decorated with bio-compatible carboxymethylated chitosan

Kianoosh Malekzadeh,1,* Rachel ghasemi goorbandi,2

1. Department of Medical Genetics; Faculty of Medicine; Hormozgan University of Medical Sciences (HUMS)
2. Molecular Medicine Research Center, Hormozgan University of Medical Sciences,

Abstract

Introduction

Genistein is an isoflavone with anti-cancer properties, but effective dosage against malignant cells was not in non-toxic range. this property impede its usage as chemotherapeutic agent. this hypothesis raised that synthesizing biocompatible nanoparticle could perhaps assist to prevail this problem.

Methods

We struggled to covalently attach genistein on fe3o4 nanoparticles decorated with carboxymethylated chitosan to fabricate a nano-vehicle in alkaline circumstance. this obtained nanoparticles were evaluated by tem, dls, ftir, xrd and vsm and its anti-cancer effect by growth rate and mtt assays as well flow cytometry of hematopoetic cancer cell lines.

Results

It presented high degree of superparamagnetic properties as well as good water solubility. significant growth inhibition of cancer cells in very lower dose of genistein nano-conjugated observed. it increased long lasting effect of genistein in cancer cells also.

Conclusion

This delivery system for genistein could be remarkably promised and futuristic as biocompatible chemotherapeutic agent against hematopoetic malignant cells.

Keywords

Acute leukemia lymphoma (all); chitosan; genistein; iron oxide nanoparticle