The efficacy of leptin on physical and psychological stress causing gastric ulcer: participations of nitric oxide and prostaglandin e2

Somaye Mansouri,1,* Afshin parviz panah,2 Mahshid sarafraz,3 Sadeq shiri,4 Soghra fallahi,5

1. - Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
2. - Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
3. - Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
4. - Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
5. Fertility and Infertility Research Center, Sharyati Hospital; Hormozgan University of Medical Sciences, Bandar Abbas, Ir

Abstract

Introduction

The present study aimed to assess the effects of leptin on physical and psychological stresses inducing gastric ulcer. the potential role of endogenous nitric oxide (no) and prostaglandin e2 (pge2) in the effects of leptin on the gastric ulcer in rats are also investigated

Methods

In this study, fifty-four male wistar rats were equally divided into 9 groups and leptin (10 µg/kg, s.c.) was administered twice a day for 14 days in advance of physical and psychological stress. also, ng- nitro-l-arginine-methyl ester (l-name, 10 mg/kg, an inhibitor of no synthase) and indomethtacin (5 mg/kg, in order to inhibit pg synthesis) were applied before leptin administration. ulcer index, gastric acid secretion, no metabolites and pge2 of stomach tissue suspension were all measured

Results

results indicated that ulcer index and gastric acid secretion were significantly decreased (p<0.05) in leptin in compare with those of single stress groups. also, no metabolites and pge2 were increased (p<0.05) in leptin groups than both l-name and indomethacin+leptin groups

Conclusion

Conclusively, data of this study emphasized the healing ability of leptin on gastric ulcer induced by physical and psychological stresses involves the no and pge2 pathways

Keywords

Leptin, gastric ulcer, nitric oxide, pge2, physical stress