The first report of akkermansia muciniphila and faecalibacterium prausnitzii abundance in obese iranian population
Fateme Ettehad marvasti,
1 Seyed davar siadat,
2,* Sara ahmadi badi,
3 Mina sadat taghavi,
4 Farzam vaziri,
5 Arfa moshiri,
6
2. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
3. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
5. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
6. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
Abstract
Introduction
The gut microbiota influences health and disease development. obesity is a multifactorial disorder that the pattern of gut microbiota has a fundamental role in its pathophysiology. intestinal anaerobic commensal bacteria such as akkermansia muciniphila and faecalibacterium prausnitzii due to their beneficial properties are very attractive to be considered in host metabolism. thus their abundance could be a potential health biomarker. since, gut microbiota pattern changes based on different criteria including ethnic, geography diet, lifestyle, age, and gender, we studied a. muciniphila and f.prausnitzii abundance in iranian obese and normal weight subjects for the first time.
Methods
Hundred normal and obese subjects recruited and classified based on body mass index (bmi).in order to determine the relative abundance of a. muciniphila and f. prausnitzii, stool samples were collected. following dna extraction from samples, qpcr was conducted based on 16s rdna universal primers.
Results
Hundred normal and obese subjects recruited and classified based on body mass index (bmi). the normal weight subjects showed the highest of a. muciniphila abundance compare to obese iranian subjects (p-value= 0.041). the abundance of f. prausnitzii did not change significantly in parallel with bmi increase (p-value= 0.204). a. muciniphila abundance significantly reduced during age increase (p-value= 0.045). the same results were not seen about f. prausnitzii (p-value= 0.173). also, the abundance of these bacteria did not have significant differences between both genders of iranian populations.
Conclusion
Because of the importance of a. muciniphila and f. prausnitzii abundance in obesity development, determination and targeted restoration of gut microbiota pattern could be valuable in the obesity treatment.
Keywords
Gut microbiota, akkermansia muciniphila,faecalibacterium prausnitzii, metabolic syndrome and obesity
