Study of mesenchymal stem cell differentiation to chondrocyte on electrospun nanofibrous polyvinyl alcohol alginate sulfat blend

Elham Taghavifar,1,* Shiva irani,2 Mohamad pezeshki-modaress3,3 Hamed daemi,4

2. 1Department of Biology, School of Basic Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.
3. 3Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran
4. Department of cell Engineering, cell science research center, royan institute for stem cell biology and technology ,ACEC

Abstract

Introduction

Cartilage tissue engineering is used as a potential solution for replacing or repairing damaged tissue. in this method, using electronically nanofibruos and providing space like the three-dimensional cell space, the cell provides field growth. the purpose of this study was to not use the growth factor on a nonfilter bed of polyvinyl alcohol and alginate sulfate polymers using electron microscopy in laboratory conditions.

Methods

In this study, scaffolds were prepared by electrospinning method using polyvinyl alcohol/alginate sulfate polymer. to evaluate the biocompatibility and proliferation of cells, mesenchymal stem cells were cultured for 72 hours using mtt assay and sem tests were done for determining of presence of cells on scaffolds.

Results

The results of electron microscopy and optical spectrometry measurements showed that polyvinyl alcohol /sulfate alginate polymer scaffold. the results of biocompatibility test showed that the cells had good proliferation and adhesion properties on the scaffold surface.

Conclusion

the polyvinyl alcohol /alginate sulfate scaffold supports the growth of mesenchymal cells and can not be considered appropriate in cartilage tissue engineering.

Keywords

Electrospin, scaffold, mesenchymal stem cell, polyvinyl alcohol, alginate sulfate