Evaluation of chelidonium majus l. alkaloidal extract effect on hela cell line compare to normal cell

Faeghe Mobaraki,1 Masoud parsania,2,* Behzad poopak,3

1. Department of Genetics,Faculty of Advanced Science and Technology,Tehran Medical Sciences,Islamic Azad University,Tehran
2. Department of Microbiology,Faculty of Medicine,Tehran Medical Sciences,Islamic Azad University,Tehran,Iran
3. Department of Laboratory Sciences, Faculty of Paramedical,Tehran Medical Sciences,Islamic Azad University,Tehran,Iran

Abstract

Introduction

Cervical cancer is one of the most common cancers in women. chelidonium majus l. is one of the most important medicinal plants in the treatment of some cancers. the aim of this study was the evaluation of the anticancer effect of the alkaloidal extract of chelidonium majus l. on hela cells with cervical cancer source.

Methods

The hela cells and fibroblast normal cells were cultured in dmem medium containing fbs and antibiotics. cells were treated with different concentrations of c.majus extract for 48 hours. mtt assay was used to evaluate the cytotoxicity of cell extracts and determine the viability. quantitative real-time pcr was used to evaluate the expression of bax and bcl-2 genes.

Results

After 48 hours, the cc50 value of the extract was determined 78 μg/ml for hela cells and 1580 μg/ml for fibroblast cells. with increasing concentration of the extract, the viability of hela cells decreased significantly compared to fibroblast cells. the results of real-time pcr showed that expression of the bax gene in hela cells that were treated with 800 μg/ml concentration of c.majus extract was significantly increased compared to 400μg/ml concentration (p =0.047). the expression of the bcl-2 gene in hela and fibroblast cells was not a significant difference (p = 0.735). the bax/bcl-2 ratio in hela cells treated with 800 μg/ml concentration was the significant difference compare to fibroblast cells (p=0.029). in this study, paired student’s t-test was used for statistical analyzes

Conclusion

In summary, the results of this study confirm that the c.majus extract induces apoptosis in hela cells in a dose depending conditions. however, it has no significant effect on the induction of apoptosis in fibroblast cells.

Keywords

Chelidonium majus l alkaloid extract, hela cell- apoptosis- bax and bcl-2.