1. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran 3. Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran
Abstract
Introduction
Hydatidosis (cystic echinococcosis, ce) as a chronic and zoonotic parasitic infection caused by the larval stage of the dog tapeworm echinococcus granulosus is still a important economic and public health difficulty all around the world especially in developing countries.
one of the treatment options for ce is surgical removal of the cysts combined with chemotherapy using albendazole and/or mebendazole before and after surgery. nowadays, several scolicidal agents, that have some aspect effects, are used for inactivation of the cyst contents. thus, the development of new scolicidal agents with low complications and more efficacies is an urgent need for surgeons. the present study was aimed to investigate the in vitro scolicidal effect of selenium nanoparticles biosynthesized by a newly isolated marine bacterial strain bacillus sp. msh-1 against protoscoleces of e. granulosus.
Methods
Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. various concentrations (50-500 mg/ml) of se nps (in size range of about 80-220 nm) were used for 10-60 min. viability of protoscoleces was confirmed by 0.1% eosin staining.
Results
The results showed that biogenic se nps at all concentrations have strong scolicidal effects especially at concentrations 500 and 250 mg/ml after 10 and 20 min of application,
respectively.
Conclusion
The findings of the present study indicated that se nps have potent scolicidal effects, therefore may be used in ce surgery. however, the in vivo efficacy of these nps remains to be explored.
