Bone marrow mesenchymal stem cells are in an environment with low oxygen pressure (hypoxia conditions)in vivo, which results in secretion of inflammatory and anti-inflammatory factors with a concentration that is several times greater than that of normomoxia. in this study, the effects of hypoxia on the expression of two factors, il-10 and tgf-β genes, which are one of the most important immunosuppressive proteins, have been studied in bone marrow and fat-derived mesenchymal stem cells the reason for choosing these two genes was their role in maintaining tolerance and preventing autoimmune diseases
Methods
:culture of bone marrow and adipose mesenchymal stem cells and studing cd90 and cd103 markers by flow cytometry, second, inducing hypoxia conditions using cobalt chloride and desfroxamine, third confirmation of hypoxia condition using hif-1a protein by western blotting, fourth, evaluation of il-10 and tgf-b gene expresiion by real time pcr (rt-pcr).
Results
: the results of microscopic examination showed that mesenchymal stem cells of bone marrow and fat were properly isolated and the results of flow cytometry confirmed microscopic results. the results of western blot confirmed the presence of hypoxic conditions in bone marrow and fatty mesenchymal cells. on the other hand, rt-pcr results confirmed the increased expression of il-10, tgf-β and vegfr anti-inflammatory factors in hypoxia compared to normoxia.
Conclusion
Given that mesenchymal cells in hypoxic conditions express more anti-inflammatory factors than those of normocoxia, they can be used in hypoxia conditions for various therapeutic programs, such as tissue transplantation and the improvement of wounds and autoimmune diseases.
Keywords
Bone marrow and fatty mesenchymal cells, hypoxia, normoxia, il-10, tgf-β and vegfr
