Study the interaction of ethylenethiourea (a metabolite of a pesticide) with c-dna via viscometric technique

Zahra Hemati,1 Soudabeh askari,2 Soheila kashanian,3,* Zohreh shariati,4

1. Amoozesh and Parvaresh, Khanmirza area, Chaharmahal and Bakhtiari
2. Biochemistry department, Biology faculty, Tarbiat Modares University, Tehran, IRAN
3. Faculty of Chemistry, Razi University, Kermanshah, IRAN
4. Biology department, Razi University, Kermanshah, IRAN

Abstract

Introduction

Study the interaction of ethylenethiourea (a metabolite of a pesticide) with c-dna via viscometric technique ethylenethiourea (etu) is an environmental degradation product, a metabolite of ethylenebisdithiocarbamate fungicides such as mancozeb, maneb and zineb. mancozeb and maneb have a wide range of approved uses on agricultural and horticultural crops in many countries. the negative effects of etu has been reported, some of these effects include altered thyroid function, effects on enzyme induction or inhibition, gene expression. while etu induces a variety of genotoxic endpoints, to our best knowledge, a few in-vitro studies exist about the interaction potential of etu with dna. this study investigated the etu effects of dna viscosity. the current study investigated the conformational change in dna induced by etu that may cause dna damages leading to genetic diseases.

Methods

The interaction of native calf thymus dna with ethylenethiourea (etu), in 10 mm hepes aqueous solutions at neutral ph=7.2, has been investigated by viscosimetric technique.

Results

The amount of 5.0 × 10-5 m dna solutions in the presence of increasing amounts of etu was used to evaluate the dna viscosity. etu induced relative increase in specific viscosity of dna.

Conclusion

In this case, there is in fact a small increase in the relative viscosity of dna by increasing the concentration of the etu complex. it has been reported [50-53] that a compound binding to dna without intercalation induces only small changes in the viscosity of dna, while drastic increments of the viscosity were observed in cases of ascertained dna intercalation by chemicals. moreover, the results obtained show that the observed small linear increase of the relative viscosity of the solution, with the increase of the etu concentration, is essentially attributable to the groove interaction through hydrogen bonding. therefore, etu can cause obvious conformational changes in the dna structure that indicates the potential toxicity of etu.

Keywords

C-dna, pesticide, ethylenethiourea, viscometry