Investigation of antioxidant and inhibitory properties of hydro alcoholic extract of eucalyptus on the production of amyloid nano biofibrils from bovine serum albumin as a model protein
1. MSc student of microbial biotechnology, Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran 2. Assistant Professor, Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract
Introduction
Alzheimer is a type of dementia that causes problems with memory, thinking and behavior. many people suffer from this illness annually. the most important factors of alzheimer’s disease are the accumulation of beta amyloid proteins and free radicals in the brain. the purpose of this investigation is analyzing the anti alzheimer’s properties of hydro alcoholic extract of eucalyptus leaf by anti amyloid nano bio fibrils and antioxidant methods.
Methods
At first, the leaves of eucalyptus were powdered and with the help of 96% ethanol, the hydro alcoholic extract of eucalyptus leaves was prepared. anti alzheimer effect of eucalyptus extract were measured with investigating by the presence of amyloid strands which done with congored spectroscopy method and by the antioxidant method which done with dpph method. notice that the dpph is a free radical.
Results
After preparing the extract with a concentration of 10 mg/ml, the anti alzheimer’s properties of eucalyptus hydro alcoholic extract were measured. the highest antioxidant activity was observed at the concentration of 9 mg/ml. the anti amyloid activity was observed at the concentration of 0.4 mg / ml of extract.
Conclusion
Due to the antioxidant and anti amyloid properties of the hydro alcoholic extract of eucalyptus leaves, this medicinal herb can be a perfect alternative for preventing and treating alzheimer’s disease.
Keywords
Anti alzheimer’s activity, amyloid nano bio fibrils, eucalyptus, hydro alcoholic extract
