Molecular characterization of polymorphism among pseudomonas aeruginosa strains isolated from burn patients wounds in shahid motahhari hospital

Parastoo Parsa,1 Mohammad ali bahar,2,* Nour amirmozafari,3 Bahareh nowruzi,4

1. Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Burn Research Center, Department of Immunology and Biology, Iran University of Medical Sciences, Tehran, Iran
3. Microbiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4. Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Pseudomonas aeruginosa is one of the most important causes of nosocomial infections. burn injuries constitute a major health concern with respect to morbidity and mortality as well as cost of management particularly in developing countries. due to the increased resistance of antibiotics, the aim of this study is to isolate pseudomonas aeruginosa strains from burn patient’s wounds by analyzing the antibiotic susceptibility and genetic profile. in this survey, we are going to find a repeatability relationship between the nucleotide pattern and antibiotic susceptibility using pcr-based rapd method.

Methods

In this research, 105 isolates of pseudomonas aeruginosa were collected from a major burn center in tehran, iran. the isolates were identified using standard biochemical tests and were examined by applying the disk diffusion and mic method to find the patterns of sensitivity and their genetic relationship was revealed through rapd-pcr technique with the help of primers 272 and 208.

Results

According to the antibiogram results, most of the isolates were resistant to 3 or more antibiotics tested and the most sensitivity was related to the colistin antibiotic. rapd-pcr method showed a high polymorphism among pseudomonas aeruginosa isolates in tehran. there was no significant correlation between the genotype groups and antibiotic susceptibility profiles.

Conclusion

We need to assess the pattern of resistance to pathogenic organisms, and identify multi-drug resistant organisms. currently, colistin antibiotic is the most suitable treatment option for burned patients. rapd-pcr is a genotyping method with a high efficiency for typifying and categorizing different isolates of mdr pseudomonas aeruginosa.

Keywords

Pseudomonas aeruginosa, rapd-pcr, nosocomial infections, burn patients