Study the effect of lactobacillus casei and cumin extract on cutaneous wound healing in wistar diabetic rats by gavage method on days 1,3,7,14,21

Atena Bazjoo,1,*

1. Department of Microbiology, Science Faculty, Islamic Azad University of Arak, Iran

Abstract

Introduction

A major metabolic disorder with increasing prevalence, diabetes can cause many medical problems. absence of a proper treatment has led to use of bioactive agents such as probiotics for treatment and prevention of this disease. this study examines the effect of lactobacillus casei as an indigenous iranian probiotic, together with cumin extract on cutaneous wound healing.in diabetic male wistar rats.

Methods

Lactobacillus casei which had high exopolysaccharide (eps) production, was selected. then, wistar male rats were divided into 2 groups: 1 group of 10 control group and one experimental group of 15 experimental groups including gavage control, experimental gavage and then rats were etherized and a square scar in the size of 1.5 × 1.5 cm on mice back was made after 24 hours of wound healing, the experimental group of pbs gavage another group were given lactobacillus casei and cumin extract.but the control group did not receive any treatment. the scar area was measured every 3 days. the skin of the killed rats on days 1,3,7,14,21 were taken and under histological and statistical studies. the results of the study were analyzed using the mean ± sd standard deviation and analyzed by anova

Results

The percentage of healing in the experimental group of gavage was daily (27.72%), (54.73%), (85.14%), (0.98%, 21), respectively. the percentage of healing in the gavage control group was 21.85 (95.21), (43.5: 5), (66.64), (92.62), respectively. the neutrophil count in the experimental group of gavage was (7.33: 3), (8.66.7), (10.33: 14), (3.75: 3) daily, respectively. neutrophile levels in the gavage control group were daily (11: 3), (7.5: 7), (4.5: 14), (25.2: 21), respectively. the amount of macrophage in the experimental group of gavage was (8: 95/8/3), (7/95/7), (4/66: 14), (1,5: 21), respectively. the amount of macrophage in the gavage control group was daily (9.9%), (83.4: 7), (66.4: 14), (21.31%), respectively. the amount of lymphocytes in the experimental group of gavages was (10.56: 3), (10.33: 7), (33.5: 14), (5.5: 21) days, respectively. the amount of lymphocytes in the gavage control group was daily (5.33: 5.3), (9.5: 7), (5.66%), (4.5: 21), respectively.

Conclusion

In this study, we observed that the probiotic bacteria consumed decreased inflammation and contributed to the process of wound healing in the gavage group.

Keywords

Cutaneous wound, exopolysaccharide, healing, lactobacillus casei, probiotic.