Protective effects of intraperitoneal administration of soybean oil on ischemia/reperfusion injury in ovaries: histological and biochemical assessments in a rat model
1. Graduated student, Faculty of veterinary Medicine , Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Abstract
Introduction
Ovarian torsion must be diagnosed and treated as much early as possible. the aim of the present study was to investigate effects of intraperitoneal administration of soybean oil on ischemia-reperfusion injury in rat ovaries.
Methods
Thirty healthy female wistar rats weighing approximately 200g were randomized into six experimental groups (n = 5): group sham: the rats were undergone only laparotomy. group i: a 3- hour ischemia only. group i/r: a 3-hour ischemia and a 3-hour reperfusion. group i/soybean oil: a 3-hour ischemia only and (0. 3 mmol/lit) intraperitoneal administration of soybean oil 2.5 hours after induction of ischemia. group i/r/soybean oil: a 3-hour ischemia, a 3-hour reperfusion and (0. 3 mmol/lit) intraperitoneal administration of soybean oil 2.5 hours after induction of ischemia. then, the ovaries were dissected out for histopathological and biochemical assessments.
Results
Soybean oil treated animals showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p<0.05). the histologic design of the ovarian tissue in the sham animals was normal. ovarian tissues in the ischemia group showed condensed hemorrhage and severe vascular congestion along with degenerative and necrotic changes in many of the cells. the tissues in the i/r group showed histopathological changes of condensed hemorrhage, infiltration of inflammatory cells along with degenerative and apoptotic cells. polymorph nuclear leukocytes (neutrophils) were dominant cell types. in i/soybean oil group general histologic and cellular structures of the tissues were not normal in appearance, however, moderate vascular congestion and edema were observed. in i/r/soybean oil group only a mild hemorrhage was around ovarian follicles. the general histologic structure of the ovarian tissue in this group was normal and no important pathologic findings in the structural level were observed except for only a mild inflammation, vascular congestion and edema. the histopathological damage were significantly lower in i/r/soybean oil animal compared to those of other groups (p<0.05). the significant higher values of sod and gst were observed in i/r/soybean oil animals compared to those of other groups (p<0.05).
Conclusion
Essentially, early diagnosis and treatment of ovarian torsion plays an important role to provide urgent protection against life-threatening complications from ischemia and to prevent future infertility. intraperitoneal administration of soybean oil could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.
Keywords
Intraperitoneal, ischemia-reperfusion, ovary, rat, soybean oil
