1. MSc student of microbiology, Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract
Introduction
Investigation on the antimicrobial effect of the herbal extracts has shown that medicinal herbs have potential resources against microbial factors. these resources have introduced new combinations from medicinal herbs. the purpose of this investigation is analyzing the antimicrobial activity of ocimum basilicum on the methicillin - resistant staphylococcus aureus and antibiotic resistant pseudomonas aeruginosa.
Methods
At first, ocimum basilicum were powdered and the hydro alcoholic extract of ocimum basilicum was prepared. antimicrobial effect of extract on the msra staphylococcus aureus and antibiotic resistant pseudomonas aeruginosa measured with disk diffusion, mic and mbc method, and amounts of mic and mbc of extract were determined. the active substances of the herb measured with using the gc-ms.
Results
The growth inhibition zone of ocimum basilicum against s.aureus and pseudomonas aeruginosa is 17 and 22 mm. the mic respectively is 25 mg/ml and 12/5 mg/ml. the mbc is respectively 50 mg/ml and 25 mg/ml for these two bacteria. the most important active substance in ocimum basilicum is stragol in gc-ms test.
Conclusion
The results showed the antimicrobial effect of hydro alcoholic extract of ocimum basilicum on the msra staphylococcus aureus and antibiotic resistant pseudomonas aeruginosa. in the future , this extract can be used as a drug against resistant bacteria.
