Investigation of the relationship between changes in expression of oncogene pi3k in acute lymphoblastic leukemia cell line jurkat e6.1 under treatment with 6mp chemotherapy drug
1. Islamic Azad university 2. Islamic Azad university 3. University of Tabriz
Abstract
Introduction
Leukemia is characterized by the excessive growth of blood cells that affects the bone marrow.acute lymphoblastic leukemia(all) is the most common type of leukemia in children and adolescents.the aim of this study was to evaluate changes in the expression of akt/pi3k signaling pathway pi3k gene in acute lymphoblastic leukemia cell line jurkat e6.1 under treatment with 6mp.
Methods
6mp was prepared at different concentrations(1-5macro molar).the jurkat e6.1 cancer cells were treated with chemotherapy after the cell passage in different groups and time (24hours).rna extraction and cdna synthesis were performed and the pi3k gene expression was evaluated by real time pcr.the results were analyzed by rest relative rest analysis software.
Results
The results of this study showed that pi3k gene in concentrations(5 macro molar) for 24 hours at concentrations showed a significant decrease(p<0.001)from the expression level of the gene.
Conclusion
Pi3k gene expression changes are influenced by the 6mp drug and decrease the expression of this oncogene in all concentrations and time,depending on time and concentration.the results of this study can be used to control and treat the optimal treatment of patients with leukemia,by identifying molecular pathways in the function of the chemotherapy drugs used,as well as introducing new drugs and preventing untreated control of jurkat cancer cells.
